ELISA Assay Diluent (5X) from BioLegend

Product Specs
ItemELISA Assay Diluent (5X)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number421203
Quantity100 mL
ApplicationsELISA
TypeSOLUTION

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral CortexInternational journal of molecular sciencesSeptember 9, 2023Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.incubation at 4 °C, wells were washed with 0.05% Tween 20 in PBS three times and 200 μL blocking solution (421203, BioLegend) was added per well for one hour. Wells were then washed and incubated for three hours with

(2) Rapid, scalable assay of amylin-β amyloid co-aggregation in brain tissue and bloodJournal of Biological ChemistryMay 1, 2023Deepak Kotiya, Noah Leibold, Nirmal Verma, Gregory A. Jicha, Larry B. Goldstein, Florin Despaof washing buffer [PBS with 0.05% of Tween 20 (0.05% PBST)] and blocked by 300 μl of assay diluent (421203, Biolegend) for 1 h at room temperature. Again, the plate was washed with 0.05% PBST washing buffer

(3) Human papillomavirus infection and seroprevalence among female university students in MexicoHuman Vaccines & ImmunotherapeuticsDecember 31, 2022Alexander Pedroza-Gonzalez et al.saline (PBS), pH 7.2 at 4°C overnight. The plates were blocked with ELISA Assay Diluent (Biolegend, Cat. 421203) at 4°C overnight. Plates were incubated with serum samples diluted 1:1000 in ELISA Assay Diluent for

(4) Development of an In-House EphA2 ELISA for Human Serum and Measurement of Circulating Levels of EphA2 in Hypertensive Patients with Renal DysfunctionDiagnostics (Basel)December 2, 2022Maki Murakoshi, Takumi Iwasawa, Takeo Koshida, Yusuke Suzuki, Tomohito Gohda, Kazunori Katoperoxidase (HRP, Cat. # 554066; Thermo Fisher Scientific, Waltham, MA, USA) in ELISA Assay Diluent (Cat. # 421203; BioLegend, San Diego, CA, USA) was added and maintained for 45 min at room temperature. Wells were

(5) Respiratory mucosal immunity against SARS-CoV-2 after mRNA vaccinationScience immunologyOctober 28, 2022Tang J, Zeng C, Cox TM, Li C, Son YM, Cheon IS, Wu Y, Behl S, Taylor JJ, Chakaraborty R, et al.washed with wash buffer (0.05% Tween 20 in PBS) and then blocked with Assay dilution buffer (Biolegend, 421203) for 1 hour at RT. Plasma or 20x concentrated BAL from unvaccinated donors, vaccinated and convalescents

(6) A Spike Protein-Based Subunit SARS-CoV-2 Vaccine for Pets: Safety, Immunogenicity, and Protective Efficacy in Juvenile CatsFrontiers in Veterinary ScienceMarch 14, 2022Kairat Tabynov, Madiana Orynbassar, Leila Yelchibayeva, Nurkeldi Turebekov, Toktassyn Yerubayev, Nurali Matikhan, Tlektes Yespolov, Nikolai Petrovsky, Kaissar TabynovCoating Buffer, #B288159, BioLegend) overnight. Plates were blocked using an ELISA Assay Diluent (#421203, BioLegend) at 200 μl per well and incubated under constant shaking (300–330 rpm on a PST-60HL thermal

(7) An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmissionNPJ vaccinesFebruary 23, 2022Tabynov K, Turebekov N, Babayeva M, Fomin G, Yerubayev T, Yespolov T, Li L, Renukaradhya GJ, Petrovsky N, Tabynov K.(ELISA Coating Buffer, #B288159, BioLegend) overnight. Plates were blocked using ELISA Assay Diluent (#421203, BioLegend) 200 µl/well and incubated under constant shaking (300-330 rpm on a PST-60HL thermal shaker,

(8) In vivo delivery of CRISPR-Cas9 using lipid nanoparticles enables antithrombin gene editing for sustainable hemophilia A and B therapyScience AdvancesJanuary 21, 2022Jeong Pil Han et al.the plate was washed four times and then blocked by adding 100 μl of assay diluent buffer (BioLegend, 421203) for 1 hour at 25°C. Sera from mice injected with LNP-CRISPR-mAT or AAV9-Cas9 were diluted 40 times,

(9) Evaluating TNF-α and Interleukin-2 (IL-2) Levels in African American Primary Open-Angle Glaucoma PatientsGenes (Basel)December 25, 2021Teja Alapati, Kyra M. Sagal, Harini V. Gudiseva, Maxwell Pistilli, Mark Pyfer, Venkata Ramana Murthy Chavali, Joan M. O’Briensimilarly. To block non-specific binding and reduce background, 200 μL Assay Diluent (Biolegend Cat #421203, Biolegend, San Diego, CA, USA) with 1X PBS containing bovine serum was added to each well. The plate

(10) Bioinformatics and Molecular Insights to Anti-Metastasis Activity of Triethylene Glycol DerivativesInternational journal of molecular sciencesJuly 30, 2020Malik V, Garg S, Afzal S, Dhanjal JK, Yun CO, Kaul SC, Sundar D, Wadhwa R.antibody (100 ng/mL) in 100 µL coating and diluent buffers (DB) (BioLegend, San Diego, CA, USA, 421701 and 421203, respectively) at room temperature overnight. Wells were washed with 200 µL 0.05% Tween 20 in PBS three

(11) Interleukin 17 and senescent cells regulate the foreign body response to synthetic material implants in mice and humansScience translational medicineApril 15, 2020Chung L, Maestas DR Jr, Lebid A, Mageau A, Rosson GD, Wu X, Wolf MT, Tam AJ, Vanderzee I, Wang X, et al.serially diluted (in the range of 1:50 to 1:102,400) in ELISA Assay Diluent (BioLegend, catalog no. 421203). Before loading, the PCL-coated plate was blocked with the assay diluent for 1 hour. After blocking,

(12) Assessment of the Sensitizing Potential of Proteins in BALB/c Mice: Comparison of Three Protocols of Intraperitoneal SensitizationNutrientsJuly 14, 2018Jesús Gilberto Arámburo-Galvez, Norberto Sotelo-Cruz, Lilian Karem Flores-Mendoza, Martina Hilda Gracia-Valenzuela, Francisco Iván Rodolfo Chiquete-Elizalde, Jesús Guadalupe Espinoza-Alderete, Humberto Trejo-Martínez, Vicente Adrián Canizalez-Román, Noé Ontiveros, Francisco Cabrera-Chávezblocking solution was discarded and 100 μL of serum samples diluted 1:10 in ELISA diluent (BioLegend, cat. 421203) were added to individual wells. After overnight incubation at 4 °C, wells were washed three times with

(13) Identification and Functional Characterization of Anti-metastasis and Anti-angiogenic Activities of Triethylene Glycol DerivativesFrontiers in oncologyJanuary 1, 2018Oh E, Garg S, Liu Y, Afzal S, Gao R, Yun CO, Kaul SC, Wadhwa R.anti-mortalin antibody diluted in coating buffer (BioLegend, 421701) and diluent buffer (Biolegend, 421203), and incubated at room temperature overnight. Wells were then washed with washing buffer (0.05% Tween

(14) Ultrasensitive isolation, identification and quantification of DNA-protein adducts by ELISA-based RADAR assayNucleic acids researchJuly 1, 2014Kiianitsa K, Maizels N.

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9/19/2019Increased Sensitivity of Homemade ELISA David TimmI'd give it a try with homemade ELISAs at least; it helped us.

ELISA Assay Diluent (5X) from BioLegend

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ELISA Assay Diluent (5X) from BioLegend

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